Article
A mutant allele skt5 affecting protoplast regeneration and killer toxin resistance has double mutations in its wild-type structural gene in Saccharomyces cerevisiae.
Bioscience, biotechnology, and biochemistry - 1 Aug 1993
Kawamoto S, Sasaki T, Itahashi S, Hatsuyama Y, Ohno T
Abstract excerpt
Saccharomyces cerevisiae strains carrying the mutant allele skt5 are resistant to the killer toxin of Kluyveromyces lactis and are defective in protoplast regeneration. The DNA sequence analysis of the cloned mutant skt5 gene showed a nucleotide substitution (causing a glycine-to-glutamic acid substitution) and also a nucleotide insertion (causing a frameshift at the extreme carboxyterminal region) in the...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Fungal
- Drug Resistance, Microbial
- Frameshift Mutation
- Fungal Proteins
- Genes, Fungal
- Killer Factors, Yeast