Article
Disruption of the gene for hsp30, an alpha-crystallin-related heat shock protein of Neurospora crassa, causes defects in thermotolerance.
Proceedings of the National Academy of Sciences of the United States of America - 23 May 1995
Plesofsky-Vig N, Brambl R
Abstract excerpt
The alpha-crystallin-related heat shock proteins are produced by all eukaryotes, but the role of these proteins in thermoprotection remains unclear. To investigate the function of one of these proteins, we disrupted expression of the single-copy hsp30 gene of Neurospora crassa, using repeat-induc...
Topics
- Cloning, Molecular
- Crosses, Genetic
- DNA, Fungal
- Escherichia coli
- Genes, Fungal
- HSP30 Heat-Shock Proteins
- Heat-Shock Proteins
- Hot Temperature
- Membrane Proteins
- Mitochondria
- Mutagenesis
- Mutagenesis, Site-Directed
- Neurospora crassa
- Phenotype
- Plasmids
- Point Mutation
- Recombinant Fusion Proteins
- Spheroplasts