Article
Analysis of enterovirus genotypes using single-strand conformation polymorphisms of polymerase chain reaction products.
Journal of virological methods - 1 Feb 1995
Fujioka S, Koide H, Kitaura Y, Duguchi H, Kawamura K
Abstract excerpt
Enterovirus genotypes were identified rapidly by reverse (RT-PCR) followed by single-strand conformation polymorphism (SSCP) analysis. The primer pair was chosen from the highly conserved sequence at the 5' non-coding region of enterovirus genomes. RT-PCR amplified a 154 bp sequence in all samples from 14 serotypes of enteroviruses, including group A and B Coxsackie viruses, echoviruses and polioviruses. SSCP...
Topics
- Base Sequence
- Conserved Sequence
- Enterovirus
- Enterovirus B, Human
- Genes, Viral
- Genotype
- Molecular Sequence Data
- Poliovirus
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
