Article
Genotyping of enteric adenoviruses by using single-stranded conformation polymorphism analysis and heteroduplex mobility assay.
Journal of clinical microbiology - 1 Apr 2004
Soares Caroline C, Volotão Eduardo M, Albuquerque Maria Carolina M, Nozawa Carlos M, Linhares Rosa Elisa C, Volokhov Dmitriy, Chizhikov Vladimir, Lu Xiaoyan, Erdman Dean, Santos Norma
Abstract excerpt
Single-stranded conformation polymorphism (SSCP) analysis and heteroduplex mobility assays (HMAs) were used to identify and genotype enteric adenoviruses (EAd). The results were compared to those of restriction endonuclease assays, species-specific PCRs, and direct nucleotide sequence analyses. Of the 31 stool samples tested, 15 isolates were identified as EAd and 7 were identified as nonenteric Ad by all...
Topics
- Adenovirus Infections, Human
- Adenoviruses, Human
- Brazil
- Child, Preschool
- DNA, Viral
- Diarrhea
- Feces
- Genotype
- Heteroduplex Analysis
- Humans
- Infant
- Infant, Newborn
- Molecular Sequence Data