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Article

Rapid molecular identification of Neisseria meningitidis isolates using the polymerase chain reaction followed by single-stranded conformation polymorphism analysis.

FEMS immunology and medical microbiology - 1 Aug 2005

Kesanopoulos Konstantinos, Tzanakaki Georgina, Velegraki Aristea, Tegos Nikolaos, Caugant Dominique A, Menounos Panagiotis, Kourea-Kremastinou Jenny, Levidiotou-Stefanou Stamatina

Abstract excerpt

Typing of Neisseria meningitidis strains is currently performed with conventional and molecular methods. Our objectives were: first, to develop a polymerase chain reaction (PCR) followed by single-stranded conformation polymorphism (SSCP) analysis of the PorA gene (VR1 region) to distinguish N. meningitidis subtypes and second, to evaluate the method for the identification and characterization of N. meningitidis...

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PMID: 16051066
DOI: 10.1016/j.femsim.2005.03.003

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