Article
Molecular basis of the complement C7 M/N polymorphism. A neutral amino acid substitution outside the epitope of the allospecific monoclonal antibody WU 4-15.
Journal of immunology (Baltimore, Md. : 1950) - 1 May 1995
Würzner R, Fernie B A, Jones A M, Lachmann P J, Hobart M J
Abstract excerpt
The allotypes of the C7 M/N polymorphism are determined by comparing the ELISA reaction pattern of the allospecific mAb WU 4-15 with polyclonal anti-C7 IgG. To characterize the molecular basis of this polymorphism, the WU 4-15 epitope was mapped by expression of cDNA fragments. It was found to be...
Topics
- Alleles
- Antibodies, Monoclonal
- Base Sequence
- Complement C7
- Electrophoresis, Polyacrylamide Gel
- Epitope Mapping
- Gene Library
- Humans
- Immunoblotting
- Isoantibodies
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Genetic