Article
Membrane vesiculation function and exocytosis of wild-type and mutant matrix proteins of vesicular stomatitis virus.
Journal of virology - 1 May 1995
Justice P A, Sun W, Li Y, Ye Z, Grigera P R, Wagner R R
Abstract excerpt
Transfection of mammalian CV1 cells with a recombinant M-gene pTM1 plasmid, driven by vaccinia virus-expressed phage T7 polymerase, resulted in the expression of matrix (M) protein, which is progressively released from the exterior surface of the transfected-cell plasma membrane. Exocytosis of M protein begins 2 to 4 h posttransfection and reaches a peak by 10 to 16 h posttransfection; dye uptake studies reveal...
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