Article
A common hot spot for somatic and germline mutations in rat mitochondrial DNA: analysis by fluorescence-based SSCP.
Biochemistry and molecular biology international - 1 Dec 1994
Kovalenko S A, Tanaka M, Borgeld H J, Ozawa T
Abstract excerpt
To estimate the degree of sequence heterogeneity of mtDNA, we have developed an efficient system for mutant detection using fluorescence-based single strand conformational polymorphism (F-SSCP). The F-SSCP analysis 200 clones from Wistar rat and sequencing of 2 clones from each 40 inbred Wistar r...
Topics
- Animals
- Base Sequence
- Cloning, Molecular
- DNA Mutational Analysis
- DNA, Mitochondrial
- Fluorescent Dyes
- Genetic Heterogeneity
- Germ-Line Mutation
- Male
- Molecular Sequence Data
- Mutation
- Polymorphism, Single-Stranded Conformational
- Rats
- Rats, Inbred Lew
- Sequence Analysis, DNA
- Sequence Homology, Nucleic Acid