Article
Characterization and analysis of a stable serotype-associated membrane protein (P30) of Mycoplasma agalactiae.
Journal of clinical microbiology - 1 Aug 2001
Fleury B, Bergonier D, Berthelot X, Schlatter Y, Frey J, Vilei E M
Abstract excerpt
The gene for a 30-kDa immunodominant antigen, P30, of Mycoplasma agalactiae was cloned from type strain PG2 and expressed in Escherichia coli. P30 is encoded on a monocistronic operon determined by two -10 boxes and a possible -35 region constituting the potential promoter, and a transcription termination site. The gene for the 266-amino-acid protein is preceded by a polypurine-rich region designed as the...
Topics
- Adhesins, Bacterial
- Animals
- Antigens, Bacterial
- Cloning, Molecular
- Genes, Bacterial
- Genetic Variation
- Molecular Sequence Data
- Mycoplasma
- Mycoplasma Infections
- Plasmids
- Recombinant Proteins
- Sequence Analysis, DNA
- Serotyping
- Sheep