Article
Rat tracheal epithelial cell differentiation in vitro.
In vitro cellular & developmental biology. Animal - 1 Jun 1993
Kaartinen L, Nettesheim P, Adler K B, Randell S H
Abstract excerpt
In vitro culture conditions enabling rat tracheal epithelial (RTE) cells to differentiate to mucociliary, mucous, or squamous phenotypes are described. Medium composition for rapid cell growth to confluence in membrane insert cultures was determined, and the effects of major modifiers of differentiation were tested. Retinoic acid (RA), collagen gel substratum, and an air-liquid interface at the level of the cell...
Topics
- Animals
- Antibodies, Monoclonal
- Cell Differentiation
- Collagen
- Culture Media
- Epithelial Cells
- Epithelium
- Immersion
- Immunohistochemistry
- In Vitro Techniques
- Keratins
- Male
