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Article

Long-term culture and characteristics of normal rat liver bile duct epithelial cells.

Gastroenterology - 1 Mar 1993

Yang L, Faris R A, Hixson D C

Abstract excerpt

BACKGROUND: A method was established for isolation and long-term culture of bile duct epithelial cells (BDEC) of normal adult rat liver that does not require the preparation of highly purified BDEC. METHODS: After dissociation of the liver parenchyma by collagenase perfusion, the liver remnant containing the intact biliary tree was minced into small fragments, embedded in a rat tail collagen gel, and cultured for...

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PMID: 7680017
DOI: 10.1016/0016-5085(93)91021-9

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