Article
Ca2+ binding to calmodulin and its role in Schizosaccharomyces pombe as revealed by mutagenesis and NMR spectroscopy.
The Journal of biological chemistry - 1 Sept 1995
Moser M J, Lee S Y, Klevit R E, Davis T N
Abstract excerpt
As a first step toward identifying the important structural elements of calmodulin from Schizosaccharomyces pombe, we examined the ability of heterologous calmodulins and Ca(2+)-binding site mutant S. pombe calmodulins to replace the essential cam1+ gene. A cDNA encoding vertebrate calmodulin all...
Topics
- Alleles
- Amino Acid Sequence
- Binding Sites
- Calcium
- Calmodulin
- Cloning, Molecular
- Escherichia coli
- Magnetic Resonance Spectroscopy
- Models, Molecular
- Mutagenesis, Site-Directed
- Phenotype
- Plasmids
- Point Mutation
- Protein Conformation
- Recombinant Proteins
- Schizosaccharomyces
- Temperature