Trypsin complexed with alpha 1-proteinase inhibitor has an increased structural flexibility.

doi:10.1016/0014-5793(95)00816-r

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Article

Trypsin complexed with alpha 1-proteinase inhibitor has an increased structural flexibility.

FEBS letters - 21 Aug 1995

Kaslik G, Patthy A, Bálint M, Gráf L

Abstract excerpt

Mutant rat trypsin Asp189Ser was prepared and complexed with highly purified human alpha 1-proteinase inhibitor. The complex formed was purified to homogeneity and studied by N-terminal amino acid sequence analysis and limited proteolysis with bovine trypsin. As compared to uncomplexed mutant try...

Topics

Amino Acid Sequence
Animals
Asparagine
Cattle
Chromatography
Electrophoresis, Polyacrylamide Gel
Humans
Molecular Sequence Data
Mutation
Protein Conformation
Rats
Serine
Trypsin
alpha 1-Antitrypsin

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