Article
A modified approach to identification of the sickle cell anemia mutation by means of allele-specific polymerase chain reaction.
Human mutation - 1 Jan 1992
Birikh K R, Plutalov O V, Schwartz E I, Devi P S, Berlin Y A
Abstract excerpt
The allele-specific PCR approach has been modified by introducing a second mismatch at the 3'-penultimate link of the primer and used to identify the sickle cell anemia mutation (A-->T transversion in the sixth codon of the human beta-globin gene causing Glu-->Val substitution in the protein), th...
Topics
- Alleles
- Amino Acid Sequence
- Anemia, Sickle Cell
- Base Sequence
- DNA
- DNA Mutational Analysis
- DNA Probes
- Evaluation Studies as Topic
- Globins
- Humans
- Molecular Sequence Data
- Point Mutation
- Polymerase Chain Reaction