Article
Mutation of Lys242 allows Delta3-Delta2-enoyl-CoA isomerase to acquire enoyl-CoA hydratase activity.
Biochimica et biophysica acta - 1 Dec 2006
Yu Wenhua, Chu Xiusheng, Deng Guisheng, Liu Xiaojun, Chen Gong, Li Ding
Abstract excerpt
We report here a novel example of generating hydratase activity through site-directed mutagenesis of a single residue Lys242 of rat liver mitochondrial Delta3-Delta2-enoyl-CoA isomerase, which is one of the key enzymes involved in fatty acid oxidation and a member of the crotonase superfamily. Lys242 is at the C-terminal of the enzyme, which is far from the active site in the crotonase superfamily and forms a...
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