Article
Evaluation of cysteine 266 of human 3-hydroxy-3-methylglutaryl-CoA lyase as a catalytic residue.
The Journal of biological chemistry - 21 Jul 1995
Roberts J R, Narasimhan C, Miziorko H M
Abstract excerpt
The role of cysteine 266 in human 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) lyase, a residue that is homologous to a cysteine mapped to the active site of prokaryotic HMG-CoA lyase by protein chemistry approaches, has been investigated by site-directed mutagenesis. Both the wild-type human enzyme...
Topics
- Amino Acid Sequence
- Binding Sites
- Cysteine
- Humans
- Kinetics
- Molecular Sequence Data
- Mutation
- Oxo-Acid-Lyases
- Structure-Activity Relationship