Article
Biochemical characterization of purified, human recombinant Lys304-->Glu medium-chain acyl-CoA dehydrogenase containing the common disease-causing mutation and comparison with the normal enzyme.
European journal of biochemistry - 1 Jun 1997
Kieweg V, Kräutle F G, Nandy A, Engst S, Vock P, Abdel-Ghany A G, Bross P, Gregersen N, Rasched I, Strauss A, Ghisla S
Abstract excerpt
Recombinant, normal human medium-chain acyl-CoA dehydrogenase (MCADH) and the common, human disease-causing K304E mutant ([Glu304]MCADH) protein were expressed in Escherichia coli using an optimized system, and the enzymes were purified to apparent homogeneity. The crucial factor leading to the p...
Topics
- Acyl-CoA Dehydrogenase
- Acyl-CoA Dehydrogenase, Long-Chain
- Catalysis
- Glutamic Acid
- Humans
- Kinetics
- Lysine
- Mutation
- Oxidation-Reduction
- Recombinant Proteins
- Substrate Specificity