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Construction and Evaluation of Plasmid Vectors Optimized for Constitutive and Regulated Gene Expression in <i>Burkholderia cepacia</i> Complex Isolates

26 Nov 2002

Abstract excerpt

Genetic studies with Burkholderia cepacia complex isolates are hampered by the limited availability of cloning vectors and by the inherent resistance of these isolates to the most common antibiotics used for genetic selection. Also, some of the promoters widely employed for gene expression in Escherichia coli are inefficient in B. cepacia. In this study, we have utilized the backbone of the vector pME6000, a...

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PMID: 12450816
DOI: 10.1128/aem.68.12.5956-5964.2002

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