Inserting the Ftz homeodomain into engrailed creates a dominant transcriptional repressor that specifically turns off Ftz target genes in vivo.

Abstract excerpt

The Engrailed homeodomain protein is an 'active' or dominant transcriptional repressor in cultured cells. In contrast, the Fushi Tarazu homeodomain protein is an activator, both in cultured cells and in Drosophila embryos, where it activates several known target genes, including its own gene. This auto-activation has been shown to depend on targeting to a fushi tarazu enhancer by the Fushi Tarazu homeodomain. We...

Topics

• Animals
• Drosophila
• Drosophila Proteins
• Enhancer Elements, Genetic
• Fushi Tarazu Transcription Factors
• Gene Expression Regulation, Developmental