Article
Characterization of pp60c-src tyrosine kinase activities using a continuous assay: autoactivation of the enzyme is an intermolecular autophosphorylation process.
Biochemistry - 14 Nov 1995
Barker S C, Kassel D B, Weigl D, Huang X, Luther M A, Knight W B
Abstract excerpt
A continuous assay for pp60c-src tyrosine kinase (srcTK) was developed. A lag in phosphorylation of the peptide RRLIEDAEYAARG was observed that could be eliminated by preincubation with MgATP. The induction time for this lag was dependent upon MgATP and srcTK concentrations. When autophosphorylat...
Topics
- Adenosine Diphosphate
- Adenosine Triphosphate
- Amino Acid Sequence
- Binding Sites
- Electrophoresis, Polyacrylamide Gel
- Enzyme Activation
- Glycerol
- Kinetics
- Magnetic Resonance Spectroscopy
- Mass Spectrometry
- Molecular Sequence Data
- Mutation
- NADP
- Oligopeptides
- Phosphorylation
- Proto-Oncogene Proteins pp60(c-src)
- Spectrophotometry