Analysis of the ion binding sites of calmodulin by electrospray ionization mass spectrometry.

doi:10.1021/bi00042a014

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Article

Analysis of the ion binding sites of calmodulin by electrospray ionization mass spectrometry.

Biochemistry - 24 Oct 1995

Lafitte D, Capony J P, Grassy G, Haiech J, Calas B

Abstract excerpt

The binding of Ca2+ and Mg2+ to four calmodulins (SynCaM 1, SynCaM 8, SynCaM 12A, and SynCaM 18A) has been studied by ESI-MS. The mass spectra were recorded by dissolving the apoproteins in methanol/water (20/80, v/v) containing 1 mM CaCl2 or 1 mM MgCl2 and the pH adjusted to 6.0 with ammonia. Th...

Topics

Amino Acid Sequence
Binding Sites
Calcium
Calmodulin
Magnesium
Mass Spectrometry
Molecular Sequence Data
Mutation

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