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Increased Production of Folate by Metabolic Engineering of <i>Lactococcus lactis</i>

1 Jun 2003

Abstract excerpt

The dairy starter bacterium Lactococcus lactis is able to synthesize folate and accumulates large amounts of folate, predominantly in the polyglutamyl form. Only small amounts of the produced folate are released in the extracellular medium. Five genes involved in folate biosynthesis were identified in a folate gene cluster in L. lactis MG1363: folA, folB, folKE, folP, and folC. The gene folKE encodes the...

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PMID: 12788700
DOI: 10.1128/aem.69.6.3069-3076.2003

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