Article
Improved sensitivity of the unscheduled DNA synthesis assay in primary rat hepatocytes following culture in serum-free defined media.
Environmental and molecular mutagenesis - 1 Jan 1995
Parton J W, Yount D J
Abstract excerpt
The unscheduled DNA synthesis (UDS) assay has been used extensively for the in vitro detection of DNA damage caused by compound exposure. However, the in vitro UDS assay has been insensitive for the detection of certain chemicals, particularly nitroaromatic compounds, that are positive in bacteri...
Topics
- Analysis of Variance
- Animals
- Autoradiography
- Cells, Cultured
- Culture Media, Serum-Free
- DNA
- DNA Damage
- Liver
- Male
- Mutagens
- Mutation
- Nitrobenzenes
- Rats
- Rats, Inbred F344
- Reference Standards
- Structure-Activity Relationship