Article
Evaluation of an automated DNA profiling system employing multiplex amplification of four tetrameric STR loci.
International journal of legal medicine - 1 Jan 1994
Kimpton C, Fisher D, Watson S, Adams M, Urquhart A, Lygo J, Gill P
Abstract excerpt
We have examined the performance and reproducibility of an automated DNA profiling system which is based on the multiplex amplification of 4 tetrameric STR loci-HUMVWFA31/A. HUMTH01, HUMF13A1 and HUMFES/FPS. The system was able to type 100 pg of purified, undegraded, genomic DNA. At lower concent...
Topics
- Alleles
- Base Sequence
- Bias
- DNA
- DNA Fingerprinting
- Electrophoresis, Polyacrylamide Gel
- Evaluation Studies as Topic
- Forensic Medicine
- Genome, Human
- Humans
- Hydrogen-Ion Concentration
- Molecular Sequence Data
- Multiphasic Screening
- Osmolar Concentration
- Polymerase Chain Reaction
- Repetitive Sequences, Nucleic Acid
- Reproducibility of Results
- Signal Processing, Computer-Assisted
