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Article

Suppression of a cold-sensitive mutation in 16S rRNA by overexpression of a novel ribosome-binding factor, RbfA.

Genes & development - 1 Mar 1995

Dammel C S, Noller H F

Abstract excerpt

A novel 15-kDa protein, RbfA, has been identified by virtue of its ability to act as a high copy suppressor of a previously characterized dominant cold-sensitive mutation (C23U) in 16S rRNA. RbfA is found associated with free 30S ribosomal subunits, but not with 70S ribosomes or polysomes, and is essential for maximal cell growth, particularly at low temperatures. Cells lacking RbfA in a wild-type rRNA background...

Topics

Amino Acid Sequence
Base Sequence
Chromosome Mapping
Cold Temperature
Escherichia coli
Escherichia coli Proteins
Gene Expression Regulation, Bacterial
Genes, Bacterial
Genetic Complementation Test
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Nucleic Acid Conformation

Phenotype

Point Mutation

RNA, Bacterial

RNA, Ribosomal, 16S

RNA-Binding Proteins

Recombinant Fusion Proteins

Ribosomal Proteins

Source

Identifiers

PMID: 7535280
DOI: 10.1101/gad.9.5.626

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7535280-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Suppression of a cold-sensitive mutation in 16S rRNA by overexpression of a novel ribosome-binding factor, RbfA.PMID 7535280Read ↗

The role of RbfA in 16S rRNA processing and cell growth at low temperature in Escherichia coli.Semantic proximity Read ↗

Mutations of ribosomal protein S5 suppress a defect in late-30S ribosomal subunit biogenesis caused by lack of the RbfA biogenesis factor.Semantic proximity Read ↗

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Suppression of a cold-sensitive mutation in ribosomal protein S5 reveals a role for RimJ in ribosome biogenesis.Semantic proximity Read ↗

The ribosome assembly gene network is controlled by the feedback regulation of transcription elongation.Semantic proximity Read ↗

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