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Article

Reliability of polymerase chain reaction (PCR) analysis of single cells for preimplantation genetic diagnosis.

Journal of assisted reproduction and genetics - 1 Feb 1994

Strom C M, Rechitsky S, Wolf G, Verlinsky Y

Abstract excerpt

PURPOSE: We investigated the reliability of polymerase chain reaction (PCR) genotype analyses performed on single cells for the purposes of preimplantation genetic analysis. METHODS: We performed blind analysis of 130 single skin fibroblasts heterozygous for the delta-F508 mutation in the cystic fibrosis transmembrane regulator (CFTR) gene and 73 single skin fibroblasts from an individual heterozygous for the...

Topics

Alleles
Amniotic Fluid
Base Sequence
Blastocyst
Cystic Fibrosis
Cystic Fibrosis Transmembrane Conductance Regulator
DNA
DNA Primers
Deoxyribonucleases, Type II Site-Specific
Diagnostic Errors

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Factor VIII

Fetal Diseases

Fibroblasts

Genetic Carrier Screening

Genotype

Humans

Membrane Proteins

Molecular Sequence Data

Source

Identifiers

PMID: 7529602
DOI: 10.1007/BF02215989

Genes

CFTR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7529602-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Reliability of polymerase chain reaction (PCR) analysis of single cells for preimplantation genetic diagnosis.PMID 7529602Read ↗

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Efficiency and accuracy of polymerase-chain-reaction assay for cystic fibrosis allele delta F508 in single cell.Semantic proximity Read ↗

Assessment of the reliability of single blastomere analysis for preimplantation diagnosis of the delta F508 deletion causing cystic fibrosis in clinical practice.Semantic proximity Read ↗

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