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Article

Efficiency and accuracy of polymerase-chain-reaction assay for cystic fibrosis allele delta F508 in single cell.

Lancet (London, England) - 16 May 1992

Liu J, Lissens W, Devroey P, Van Steirteghem A, Liebaers I

Abstract excerpt

Diagnosis of genetic disorders in the embryo before implantation, though possible by removal of one or two blastomeres at the eight-cell stage, is still experimental because the procedures of gene analysis of DNA from a single cell are not yet reliable enough for clinical application. We have eva...

Topics

Alleles
Cystic Fibrosis
Embryonic Development
Female
Heterozygote
Humans
Male
Polymerase Chain Reaction
Pregnancy
Prenatal Diagnosis
Spermatozoa

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 1349937
DOI: 10.1016/0140-6736(92)91133-s

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1349937-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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Efficiency and accuracy of polymerase-chain-reaction assay for cystic fibrosis allele delta F508 in single cell.PMID 1349937Read ↗

Polymerase chain reaction analysis of the cystic fibrosis delta F508 mutation in human blastomeres following oocyte injection of a single sperm from a carrier.Semantic proximity Read ↗

Assessment of the reliability of single blastomere analysis for preimplantation diagnosis of the delta F508 deletion causing cystic fibrosis in clinical practice.Semantic proximity Read ↗

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Detection of both the normal and mutant alleles in single cells of individuals heterozygous for the sickle cell mutation--prelude to preimplantation diagnosis.Semantic proximity Read ↗

Allelic drop-out and preferential amplification in single cells and human blastomeres: implications for preimplantation diagnosis of sex and cystic fibrosis.Semantic proximity Read ↗

Reliability of polymerase chain reaction (PCR) analysis of single cells for preimplantation genetic diagnosis.Semantic proximity Read ↗

Increasing the denaturation temperature during the first cycles of amplification reduces allele dropout from single cells for preimplantation genetic diagnosis.Semantic proximity Read ↗

Detailed investigation of factors influencing amplification efficiency and allele drop-out in single cell PCR: implications for preimplantation genetic diagnosis.Semantic proximity Read ↗

Direct comparison of detection systems used for the development of single-cell genetic tests in preimplantation genetic diagnosis.Semantic proximity Read ↗

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