Article
Solution structure of FK506 bound to the R42K, H87V double mutant of FKBP-12.
Biochemistry - 22 Nov 1994
Lepre C A, Pearlman D A, Cheng J W, DeCenzo M T, Livingston D J, Moore J M
Abstract excerpt
The binding of the FK506/FKBP-12 complex to calcineurin (CN), its putative target for immunosuppression, involves recognition of solvent-exposed regions of the ligand as well as FKBP-12 residues near the active site. The R42K, H87V double mutation of FKBP-12 decreases the CN affinity of the compl...
Topics
- Calcineurin
- Calmodulin-Binding Proteins
- Carrier Proteins
- Computer Graphics
- Computer Simulation
- Crystallography, X-Ray
- Heat-Shock Proteins
- Humans
- Magnetic Resonance Spectroscopy
- Mutation
- Phosphoprotein Phosphatases
- Protein Binding
- Protein Conformation
- Recombinant Proteins
- Solutions
- Tacrolimus
- Tacrolimus Binding Proteins