Article
Occurrence of N-acetyl- and N-O-diacetyl-neuraminic acid derivatives in wild and mutant Crithidia fasciculata.
Parasitology research - 1 Jan 1995
Matta M A, Aleksitch V, Angluster J, Alviano C S, De Souza W, Andrade A F, Esteves M J
Abstract excerpt
The cell-surface expression of sialic acids in wild-type Crithidia fasciculata and three drug-resistant mutants (FU(R)11, TR3, and TFRR1) was analyzed using fluorescein-labeled Limulus polyphemus agglutinin (LPA) binding, glycosidase of known sugar specificity, and thin-layer chromatography (TLC). Gas-liquid chromatography-mass spectrometry (GC-MS) analysis using both electron-impact (EI-MS) and chemical...
Topics
- Animals
- Arthropod Proteins
- Chromatography, Thin Layer
- Crithidia fasciculata
- Fluorescein-5-isothiocyanate
- Gas Chromatography-Mass Spectrometry
- Lectins
- Mutation
- N-Acetylneuraminic Acid
- Sialic Acids