Article
Mutants in a yeast Ran binding protein are defective in nuclear transport.
The EMBO journal - 1 Nov 1995
Schlenstedt G, Wong D H, Koepp D M, Silver P A
Abstract excerpt
Ran, a Ras-like GTPase, has been implicated in controlling the movement of proteins and RNAs in and out of the nucleus. We have constructed strains of Saccharomyces cerevisiae which produce fusion proteins containing glutathione-S-transferase (GST) fused to Gsp1p, which encodes the essential yeast Ran homolog, and a mutant form of Gsp1p that mimics the GTP-bound state. A major protein with the apparent size of 34...
Topics
- Amino Acid Sequence
- Base Sequence
- Biological Transport, Active
- Cell Nucleus
- Fungal Proteins
- GTP-Binding Proteins
- Molecular Sequence Data
- Mutation
- Nuclear Proteins
- RNA
- Recombinant Fusion Proteins