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Article

Use of antibiotics to select auxotrophic mutants of Neisseria meningitidis.

Microbial pathogenesis - 1 Apr 1995

Erwin A L, Gill M J, Gotschlich E C

Abstract excerpt

Antibiotic selection of auxotrophs has been a powerful tool in the elucidation of bacterial metabolic pathways, but it has been difficult to adapt this method to Neisseria spp. We describe a procedure by which a population of mutagenized N. meningitidis is enriched for mutants with specific growth phenotypes. These experiments used a simple defined medium (modified from that described in J Bacteriol 1962; 83:...

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PMID: 7476094
DOI: 10.1016/s0882-4010(05)80005-1

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