Laboratory diagnosis of gonorrhea by a simple transformation test with a temperature-sensitive mutant of Neisseria gonorrhoeae.

doi:10.1097/00007435-198010000-00008

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Article

Laboratory diagnosis of gonorrhea by a simple transformation test with a temperature-sensitive mutant of Neisseria gonorrhoeae.

Sexually transmitted diseases - 1 Jan 2000

Zubrzycki L, Weinberger S S

Abstract excerpt

A temperature-sensitive mutant of Neisseria gonorrhoeae strain tsA-1 was used in a transformation test for the laboratory diagnosis of gonorrhea. This transformation test (C test) is based on a spotting of a DNA lysate, obtained through simple base-acid extraction of a cervical-swab specimen, ont...

Topics

Bacteriological Techniques
Cervix Mucus
Culture Media
DNA, Bacterial
Female
Gonorrhea
Humans
Mutation
Neisseria gonorrhoeae
Temperature
Transformation, Genetic
Vaginal Smears

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