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Article

Cloning and characterization of the catalase gene of Neisseria gonorrhoeae: use of the gonococcus as a host organism for recombinant DNA.

Infection and immunity - 1 Jul 1996

Johnson S R, Steiner B M, Perkins G H

Abstract excerpt

The structural gene for the catalase of Neisseria gonorrhoeae was cloned into a Kat- strain of that organism by using a recombinant vector derived from one of the beta-lactamase-specifying plasmids found in that organism. The kat gene was then successfully subcloned into both pUC8 and pGB2, transformed into Escherichia coli, and shown to complement the E. coli katE mutants UM2 and UMRl. The gene was subsequently...

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Identifiers

PMID: 8698488
DOI: 10.1128/iai.64.7.2627-2634.1996
PMCID: PMC174119

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