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Article

Tn5 mutagenesis of the transforming genes of human adenovirus type 5.

Gene - 1 Jan 2000

McKinnon R D, Bacchetti S, Graham F L

Abstract excerpt

We have cloned the entire human adenovirus type 5 (Ad5) genome into the pBR322 plasmid in two segments: the BamHI-A fragment (21 kb) and the BamHI-B fragment (15 kb). We have also generated a series of clones with smaller Ad5 DNA inserts, all containing the left-end of the viral genome. One such clone, pXCl, containing the left 16% of the Ad5 DNA molecule, has been shown to transform rodent cells by DNA...

Topics

Adenoviruses, Human
Animals
Cell Transformation, Viral
Cells, Cultured
Cloning, Molecular
Cricetinae
DNA, Recombinant
DNA, Viral
Escherichia coli
Genes, Viral
Kidney
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Plasmids

Rats

Source

Identifiers

PMID: 6754531
DOI: 10.1016/0378-1119(82)90186-x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-6754531-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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