Article
[Construction of vectors for eukaryotes on the basis of fragments of mitochondrial DNA of animals].
Voprosy meditsinskoi khimii - 1 Jan 2000
Kazakova T B, Babich S G, Golovina G I, Nevzgliadova O V, Smolianitskiĭ A G
Abstract excerpt
The Bam HI fragment of rat liver mtDNA was used for construction and analysis of the cloning vehicles for prokaryota and eukaryota. Transformation of mutant bacterial cells deficient in DNA polymerase I polA with recombinant pBR-mtBA DNA was shown previously. Now the transformation of bacterial cells with recombinant pmt Tn9 DNA was observed. The Bam HI-A fragment of animal mtDNA may be used as vehicle replicon...
Topics
- Animals
- Bacteria
- Cloning, Molecular
- DNA Polymerase I
- DNA, Mitochondrial
- DNA, Recombinant
- Liver
- Mutation
- Plasmids
- Rats
- Saccharomyces cerevisiae