Article
[Comparative analysis of the kinetics of renaturation of newly synthetized and total DNA of eukaryotes as a means of demonstrating selective genome synthesis].
Molekuliarnaia biologiia - 1 Jan 2000
Romanov G A, Vaniushin B F
Abstract excerpt
A simple and sensitive method for determining the selective eucaryotic DNA synthesis (amplification, ordered replication) amounting up to 10(-6)% of genome has been proposed. The essence of the method is measuring of the combined renaturation of total and in vivo labelled newly synthesized DNA. By means of a special computer program a theoretical investigation of amplification (from 50 to 2-fold) was...
Topics
- Animals
- DNA
- Genotype
- Hydrocortisone
- Kinetics
- Liver
- Nucleic Acid Renaturation
- Rats