Article
Properties and kinetics of development of Rous sarcoma virus-infected cells evidenced by methylene blue staining.
Intervirology - 1 Jan 2000
Barlati S, Mignatti P, Kryceve C, Vigier P
Abstract excerpt
Three different kinds of areas of infected cells corresponding to different focus formation stages can be evidenced by methylene blue (MB) staining in cultures of chick embryo (CE) fibroblasts infected at low multiplicity with the temperature-sensitive (ts) mutant of Rous sarcoma virus (RSV), FU19, which transforms these fibroblasts at 37 degrees but not at 41 degrees. These are: (a) areas of MB-stainable cells...
Topics
- Animals
- Avian Sarcoma Viruses
- Cell Transformation, Neoplastic
- Chick Embryo
- Culture Techniques
- DNA
- DNA, Neoplasm
- Kinetics
- Methylene Blue
- Mutation
- RNA
- RNA, Neoplasm
