Article
Replication-defective mutants of mouse cytomegalovirus protect against wild-type virus challenge.
Journal of medical virology - 1 Oct 2000
Gill T A, Morley P J, Sweet C
Abstract excerpt
Five temperature-sensitive mutants (tsm9, tsm13, tsm20, tsm22, tsm30) of murine cytomegalovirus have been shown previously not to produce infectious virus in mice. In the present study, the stage at which these mutants are blocked in their replication in vitro was examined by transcriptional analysis of 4 temporally regulated marker genes (IE-1, E-1, gB and gH) using a semi-quantitative reverse transcription...
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