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Article

High Resolution Genotyping of Leptospira Direct from Clinical Samples Using Multiplexed Amplicon Sequencing.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2026

Stone Nathan E, Sahl Jason W

Abstract excerpt

Amplicon sequencing (AmpSeq) is a high-throughput, PCR-based sequencing approach for high-resolution and inexpensive genotyping. We designed and validated a 42 locus AmpSeq system for strain-level Leptospira genotyping directly from complex clinical samples such as urine, blood, or animal tissues.

Read the complete abstract on PubMed

Topics

Leptospira
Humans
Genotyping Techniques
Genotype
High-Throughput Nucleotide Sequencing
Animals
Leptospirosis
Multiplex Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

DNA, Bacterial

Phylogeny

Polymerase Chain Reaction

Source

Identifiers

PMID: 42681480
DOI: 10.1007/978-1-0716-5500-9_4

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 24 Oct 2026.

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