Preanalytical stability of hemoglobin H inclusion body detection using rapid brilliant cresyl blue staining: a genotype-stratified stability study in EDTA blood stored at 4 °C.

Abstract excerpt

INTRODUCTION: No evidence-based preanalytical guideline exists for EDTA blood storage before hemoglobin H (HbH) inclusion body detection. We evaluated the stability of rapid (30-minute) brilliant cresyl blue staining across α-globin and β-globin genotypes in blood stored at 4 °C. METHODS: Residual EDTA blood from 322 patients with microcytic hypochromic anemia was stored at 4 °C and tested on days 0, 2, 4, and 7...

Topics

• Humans
• Edetic Acid
• Genotype
• Hemoglobin H
• Staining and Labeling
• Inclusion Bodies