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Article

ChIP-MS in Plant Systems: Mapping the H3K27ac Proteome During the Greening Process.

Physiologia plantarum - 1 Jan 2026

Brun Alexis, Quevedo Marti, Sterling Luis A, Dekkers Dick H W, Demmers Jeroen, Hudson Elton Paul, Strand Åsa

Abstract excerpt

We have established a method for chromatin immunoprecipitation coupled to mass spectrometry (ChIP-MS) in Arabidopsis thaliana. We demonstrate its utility by investigating proteins associated with histone H3 lysine 27 acetylation (H3K27ac), a key epigenetic mark regulating photosynthesis-associated nuclear genes (PhANGs) during chloroplast development and establishment of photosynthesis. Purification of...

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PMID: 41706575
DOI: 10.1111/ppl.70797
PMCID: PMC12915887

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