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Article

CRISPRi targeting to the Neisseria gonorrhoeae R-loop or G4 mutant region restores pilin antigenic variation.

mBio - 12 Aug 2026

Metaane Selma, Seifert H Steven

Abstract excerpt

Neisseria gonorrhoeae pilin antigenic variation (pilin Av) is a complex diversity generation system. Pilin Av depends on a Rec-dependent gene conversion event initiated by an R-loop (gar sRNA) and a resultant G-quadruplex (G4) DNA structure. Mutations of the garP promoter or the G4-forming sequence each prevent pilin Av. We targeted a Type I-C CRISPR interference (CRISPRi) complex to the mutant garP or G4 loci,...

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Identifiers

PMID: 42405794
DOI: 10.1128/mbio.01347-26
PMCID: PMC13463697

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