Evaluation of bone preparation approaches using length-based analysis and targeted sequencing for forensic human identification of historic skeletal remains.

Abstract excerpt

Advances in DNA technology have significantly enhanced the forensic community's ability to develop genetic profiles from unidentified human skeletal remains. However, sampling requires mechanical grinding of hard tissues before DNA isolation. This processing can compromise genetic profiles, particularly in aged bones. We compared the industry-standard pulverization method with an alternative powder-free...

Topics

• Humans
• DNA Fingerprinting
• DNA
• Microsatellite Repeats
• Polymorphism, Single Nucleotide
• High-Throughput Nucleotide Sequencing