Article
Evaluation of the performance of reverse transcription-recombinase polymerase amplification (RT-RPA) coupled with CRISPR/Cas12a and microfluidics for one-step detection of common HCV genotypes.
Diagnostic microbiology and infectious disease - 1 Nov 2026
Wang Qiong, Wang Yushu, Jia Tian, Hu Xiaodong, Chen Fuliang
Abstract excerpt
BACKGROUND: Hepatitis C virus (HCV) genotyping is critical for guiding therapy, yet current methods are technically demanding and time-consuming. AIM: To develop and evaluate a one-step, integrated assay combining reverse transcription-recombinase polymerase amplification (RT-RPA) with CRISPR/Cas12a detection on a microfluidic platform for rapid and accurate HCV genotyping. METHODS: The assay was validated using...
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