Article
Functional interaction surfaces of the bacterial transcription terminator Rho are required for the interactions with the elongation factor NusG.
The Biochemical journal - 27 May 2026
Behera Abhijeet, Chhakchhuak Passong Immanual R, Jain Sriyans, Sen Ranjan
Abstract excerpt
Transcription terminator Rho interacts with the elongation factor NusG to induce efficient termination. By employing random mutagenesis, structure-guided site-directed mutagenesis, and phenotypic re-evaluations of our laboratory mutants, we identified several mutations in the Rho-linker region (∼135-155) and the hydrophobic pocket (200-230), in addition to those observed in the C-terminal domain (CTD) region...
Topics
- Rho Factor
- Transcription Factors
- Peptide Elongation Factors
- Escherichia coli Proteins
- Protein Binding
- Escherichia coli
- Mutagenesis, Site-Directed
- Mutation
- Protein Domains