Article
Analysis of E. coli rho factor: mutations affecting secondary-site interactions.
Journal of molecular biology - 4 Aug 1995
Pereira S, Platt T
Abstract excerpt
To define and differentiate primary and secondary RNA binding sites within the linear sequence of the rho protein, we investigated two mutant alleles, rho-115 and rhosuA1. They were first identified as defective in transcription termination in vivo, and later demonstrated to be defective in their interactions with RNA at the primary and secondary sites, respectively. Sequencing of rhosuA1 revealed a single lysine...
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