Manual validation finds ultra-long-read sequencing best enables faithful, population-level structural variant calling in Drosophila melanogaster euchromatin with nanopore.

Abstract excerpt

The increasing accessibility of long-read sequencing and the rapid development of automated variant callers are promoting the generation of population-level structural variation data. However, the effect of the length of long-reads on automated variant callers is not well understood, especially for non-human species. Here we show that only ultra-long long-reads, with read N50s greater than 50 kb, are capable of...

Topics

Join the communities discussing this publication.

• Drosophila melanogaster
• Euchromatin
• Genomic Structural Variation
• Genomics
• High-Throughput Nucleotide Sequencing