Protocols to evaluate mutant specificity of an oncogene-targeting siRNA using orthogonal in vitro and in vivo approaches.

Abstract excerpt

RNA interference (RNAi) is a promising new approach for oncogene targeting for "undruggable" targets, including KRAS. Here, we present a protocol for evaluating mutant selectivity of KRAS small interfering RNAs (siRNAs) using orthogonal in vitro and in vivo techniques. We describe steps for structural analyses of siRNA complexes, utilization of isogenic HA- and luciferase-tagged cell lines, RNA sequencing for...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to KRAS gene
• Share to Mutation
• Share to Oncogenes