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Swift SNPs: Evaluating Rapid DNA Extraction Methods for Scalable Genotyping in Aquaculture.

Marine biotechnology (New York, N.Y.) - 9 Jan 2026

Rhodes Emily, Jerry Dean, Jones David B, Goldsbury Julie, Vu Nga

Abstract excerpt

Efficient DNA extraction is critical for high-throughput genotyping in aquaculture breeding programs, where cost, speed, and data quality must be balanced. This study compared five rapid extraction methods-HotSHOT, Chelex, Tween, wax-coated dipstick, and bare dipstick-against a conventional cetyltrimethylammonium bromide (CTAB) protocol for use in Lates calcarifer (barramundi) genotyping. DNA yield, purity, and...

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PMID: 41511667
DOI: 10.1007/s10126-025-10561-7
PMCID: PMC12789216

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