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Fast and Accurate Enrichment of Canid Excrement for Species* and Other Analyses (FAECES*) Method for Noninvasive Genotyping of Field-Collected Canid Scat.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2025

Parker Lillian D, Maldonado Jesús E, Campana Michael G

Abstract excerpt

The FAECES protocol details a method for obtaining multi-locus SNP genotypes from noninvasively collected scat samples. It employs a capture enrichment technique that can be adapted for various vertebrate species. The workflow encompasses probe design using BaitsTools, DNA extraction using the Qiagen DNeasy PowerSoil Pro kit, genomic DNA library preparation via the Santa Cruz Reaction (SCR) protocol, in-solution...

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PMID: 40580297
DOI: 10.1007/978-1-0716-4642-7_17

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